Abstract: In view of the problems existed in the process of resource utilization and energy utilization of kitchen waste, continuous anaerobic fermentation experiments were conducted at medium temperatures (38 ℃) and intermediate temperatures (44 ℃) to analyze the gas production characteristics, process parameters, microbial colony structure and composition of different temperature fermentation systems. The results showed that under the same organic load, compared to medium temperatures experimental group, the intermediate temperatures experimental group had biogas production characteristics such as higher volumetric biogas production rate and methane production rate. And it had a high degradation rate of VS. The intermediate temperatures fermentation system was more stable in the analysis of process parameters such as VFAs, ammonia nitrogen, total alkalinity, pH, etc, and it had a certain resistance to load impact. In the exploration of high-throughput microbial community diversity, intermediate temperatures fermentation was characterized by a relatively abundance diversity of bacterial species, and archaea had obvious superiority in producing methane, which indicated that during the anaerobic fermentation process, the mutual promotion and coordination between microbial colonies in the intermediate temperatures anaerobic fermentation system promoted the stable and orderly transformation of various material parameters, and guaranteed the stable and efficient operation of the anaerobic system.

Key words: kitchen waste, anaerobic fermentation, intermediate temperatures, medium temperatures, microbial community