Abstract: Taking kitchen waste collected by classification as fermentation substrate， and taking wet anaerobic sludge and cow manure as inoculums respectively，the effect of different inoculums on anaerobic digestion of kitchen waste was investigated under the condition of medium temperature was（38±1） ℃ and the inoculation ratio between inoculums and kitchen waste was 2∶1．The results showed that wet anaerobic sludge of food waste had better inoculation effect than that from cow manure in cattle farm. The inoculation effect of the digested sludge in the wet anaerobic sludge was the best， and the peak gas production per day was achieved on the third day of the fermentation. Moreover， its cumulative gas production was the highest with 1619 mL， and the VS degradation rate was 21.7%.